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Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes

Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes

DOI:
10.3791/56802-v

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12:46 min

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December 06, 2017

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,
1Department of Biological Sciences,Dartmouth College

Capitoli

  • 00:05Titolo
  • 00:50Removal of Chorions and Vitelline Membranes
  • 05:57FISH
  • 10:27Results: Representative Images of Meiotic Figures with Intact Arm Cohesion and Disrupted Arm Cohesion
  • 11:31Conclusion

Summary

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This manuscript presents a detailed method for generating X-chromosome arm probes and performing fluorescence in situ hybridization (FISH) to examine the state of sister chromatid cohesion in prometaphase and metaphase I arrested Drosophila oocytes. This protocol is suitable for determining whether meiotic arm cohesion is intact or disrupted in different genotypes.

Tags

Keywords: Fluorescence In Situ Hybridization (FISH)Drosophila OocytesPrometaphase IMetaphase IMeiotic Arm CohesionMaternal Age EffectOocyte RollingPBSBTXFrosted Glass Slides

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