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利用荧光原位杂交 (fish) 监测中期和中期 I. 型果蝇卵母细胞的臂部凝聚状态
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes
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利用荧光原位杂交 (fish) 监测中期和中期 I. 型果蝇卵母细胞的臂部凝聚状态

DOI:
10.3791/56802-v

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12:46 min

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December 06, 2017

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1Department of Biological Sciences,Dartmouth College

Capitoli

  • 00:05Titolo
  • 00:50Removal of Chorions and Vitelline Membranes
  • 05:57FISH
  • 10:27Results: Representative Images of Meiotic Figures with Intact Arm Cohesion and Disrupted Arm Cohesion
  • 11:31Conclusion

Summary

Traduzione automatica

Traduzione automatica

本手稿提出了一个详细的方法生成X-染色体臂探针和执行荧光原位杂交 (鱼), 以检查中期和中期我被捕的姐妹染色单体的内聚力状态果蝇卵母细胞。本协议适用于确定不同基因型的减数分裂手臂的内聚力是否完好或中断。

Tags

Keywords: Fluorescence In Situ Hybridization (FISH)Drosophila OocytesPrometaphase IMetaphase IMeiotic Arm CohesionMaternal Age EffectOocyte RollingPBSBTXFrosted Glass Slides

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