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Journal / Immunology and Infection / A Co-culture Method to Investigate the Crosstalk…
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Immunology and Infection

This Contenuto is Open Access.

JoVE Journal Immunology and Infection
A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC
 

A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC

Article DOI: 10.3791/56908-v • 11:40 min • January 30th, 2018
January 30th, 2018 •
*1, *1, 1, 1, 2, 2, 2, 1
1Dipartimento di Fisica, Università degli Studi di Pavia, 2IRCCS Istituti Clinici Scientifici Maugeri
* These authors contributed equally

Capitoli

Riepilogo
Automatic Translation

We present a protocol to investigate the crosstalk between X-ray-irradiated Caco-2 and peripheral blood mononuclear cells (PBMC). The protocol starts with Caco-2 irradiation and set-up of the co-culture with PBMC; subsequently, trans-epithelial electrical resistance is measured regularly over 48 h and western blot performed in both Caco-2 and PBMC.

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Keywords: Caco-2 Cells PBMCs Coculture Ionizing Radiation Epithelial Cell Permeability Tight Junction Function Radiation Biology Radioimmunotherapy Cell Culture Density Gradient Centrifugation X-ray Irradiation Dosimetry Cell Viability
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