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Genetics

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JoVE Journal Genetics
A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells
 

A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells

Article DOI: 10.3791/56915-v • 10:42 min • December 12th, 2017
December 12th, 2017 •
1, 1
1Duke Viral Vector Core, Department of Neurobiology, Duke University School of Medicine

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We describe the production strategy of integrase-deficient lentiviral vectors (IDLVs) as vehicles for delivering CRISPR/Cas9 to cells. With an ability to mediate quick and robust gene editing in cells, IDLVs present a safer and equally effective vector platform for gene delivery compared to integrase-competent vectors.

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Keywords: Integrase-deficient Lentiviral Vectors CRISPR/Cas9 Gene Editing HEK-293T Cells Transfection Sucrose Gradient Ultracentrifugation
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