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Cancer Research

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JoVE Journal Cancer Research
A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells
 

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

Article DOI: 10.3791/56932-v • 11:15 min • May 6th, 2018
May 6th, 2018 •
*1, *2, 2
1Neuro-oncology Research Group, VU Medical Center, 2Exosomes Research Group, Department of Pathology, VU Medical Center
* These authors contributed equally

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Direct injection of cancer-derived extracellular vesicles (EVs) leads to reprogramming of bone marrow supporting tumor progression; however, which cells mediate this effect is unclear. Herein, we describe a step-by-step protocol to investigate EV-mediated tumor-mesenchymal stem cell (MSC) interactions in vivo, revealing a crucial role for EV-educated MSCs in metastasis.

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Preclinical Mouse Model Osteosarcoma Extracellular Vesicles Communication Tumor Mesenchymal Stem Cells Cancer Biology Cancer Progression Adaptation Metastasis Formation Therapy GSA EV Communication Androgen Signaling Inhibition Osteosarcoma Bone Marrow Cancer-supporting Cells Seeding Flasks 143B Cells EV-depleted Medium Supernatants Isolation Differential Centrifugation
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