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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation and Characterization of Neutrophil-derived Microparticles for Functional Studies
 

Isolation and Characterization of Neutrophil-derived Microparticles for Functional Studies

Article DOI: 10.3791/56949-v • 06:56 min • March 2nd, 2018
March 2nd, 2018 •
1, 1, 1, 1
1Department of Pathology, Feinberg School of Medicine, Northwestern University

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New protocols are described here to isolate and characterize microparticles derived from human and mouse neutrophils. These protocols utilize ultracentrifugation, flow cytometry, and immunoblotting techniques to analyze microparticle content, and they can be used to study the role of microparticles derived from various cell types in cellular function.

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Keywords: Neutrophil-derived Microparticles Cell-cell Communication Density Gradient Separation Polymorphonuclear Cells PMN Mononuclear Cells Cell-free Supernatants Ultra-centrifugation Functional Studies
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