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Journal / Immunology and Infection / Isolation of Functional Cardiac Immune Cells
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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation of Functional Cardiac Immune Cells
 

Isolation of Functional Cardiac Immune Cells

Article DOI: 10.3791/3020-v • 07:27 min • December 5th, 2011
December 5th, 2011 •
1, 1, 1, 1, 1, 1
1Department of Cell Biology and Anatomy, University of South Carolina- School of Medicine

Capitoli

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This method for isolating functional immune cells from the heart provides an alternative to the conventional methods of collagenase digestion, which causes unwanted immune cell activation, resulting in a decreased responsiveness of these cells. Our method of isolation yields functional cardiac immune cells by avoiding problems associated with enzymatic digestion.

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Functional Cardiac Immune Cells Pathological Remodeling Cardiac Diseases Mast Cells T-cells Macrophages Biologically Active Mediators Cytokines Proteases Tryptase Extracellular Matrix Metabolism Matrix Metalloproteinases Collagen Accumulation Cardiac Fibroblasts' Function Isolating Cardiac Immune Cells Myocardial Tissue Digestion Loss Of Function Technique Development Understanding Of Role In Cardiac Disease Anatomical Location Rat's Xiphoid Process Axilla Falciform Ligament Pericardium Of The Heart Characterization Of Functionality Phenotype Maturity
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