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Immunologia e infezioni

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人乳样品靶向16S 测序方法的研究

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

A Method for Targeted 16S Sequencing of Human Milk Samples

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人乳样品靶向16S 测序方法的研究

DOI:

10.3791/56974-v

•

09:09 min

•

March 23, 2018

•

Nicole H. Tobin, Cora Woodward, Sara Zabih, David J. Lee, Fan Li, Grace M. Aldrovandi

¹Department of Pediatrics,University of California at Los Angeles

Capitoli

00:04Titolo
00:37Sample Preparation
01:41Sample Disruption and Homogenization
04:37Preparing the Robotic Liquid Handler for Automated Targeted 16S PCR Setup
06:50Results: Targeted 16S Sequencing of Human Milk Samples
08:23Conclusion

Summary

Traduzione automatica

提出了一种半自动化工作流, 用于从人乳和其他低生物量样品类型中进行 16S rRNA 的目标排序。

Tags

16S Sequencing Human Milk Low Biomass Samples Semi-automated Protocol Microbiome Cell Pellet Lysis Buffer Bead Beating DNA Extraction PCR Amplification

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