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Immunology and Infection

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A Method for Targeted 16S Sequencing of Human Milk Samples

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Immunology and Infection

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JoVE Journal Immunology and Infection

A Method for Targeted 16S Sequencing of Human Milk Samples

A Method for Targeted 16S Sequencing of Human Milk Samples

DOI:

10.3791/56974-v

•

09:09 min

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March 23, 2018

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Nicole H. Tobin, Cora Woodward, Sara Zabih, David J. Lee, Fan Li, Grace M. Aldrovandi

¹Department of Pediatrics,University of California at Los Angeles

Chapters

00:04Title
00:37Sample Preparation
01:41Sample Disruption and Homogenization
04:37Preparing the Robotic Liquid Handler for Automated Targeted 16S PCR Setup
06:50Results: Targeted 16S Sequencing of Human Milk Samples
08:23Conclusion

Summary

Automatic Translation

A semi-automated workflow is presented for targeted sequencing of 16S rRNA from human milk and other low-biomass sample types.

Tags

16S Sequencing Human Milk Low Biomass Samples Semi-automated Protocol Microbiome Cell Pellet Lysis Buffer Bead Beating DNA Extraction PCR Amplification

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