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Journal / Genetics / Embryo Microinjection and Transplantation…
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Genetics

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JoVE Journal Genetics
Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation
 

Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation

Article DOI: 10.3791/56990-v • 09:05 min • December 25th, 2017
December 25th, 2017 •
1,2, 1,2, 1,2
1Department of Entomology and Riverside Center of Disease Vector Research, Institute for Integrative Genome Biology, University of California, Riverside, 2Section of Cell and Developmental Biology, Division of Biological Sciences, University of California, San Diego

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Microinjection of Nasonia vitripennis embryos is an essential method for generating heritable genome modifications. Described here is a detailed procedure for microinjection and transplantation of Nasonia vitripennis embryos, which will greatly facilitate future genome manipulation in this organism.

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Keywords: Embryo Microinjection Nasonia Vitripennis Genome Manipulation Venom Production Sex Determination Wasp Colonies Blowfly Pupae Parasitization Embryo Collection Embryo Alignment Slide Microinjection Technique
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