Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / Standardized Measurement of Nasal Membrane…
JoVE Journal
Medicine

This Contenuto is Open Access.

JoVE Journal Medicine
Standardized Measurement of Nasal Membrane Transepithelial Potential Difference (NPD)
 

Standardized Measurement of Nasal Membrane Transepithelial Potential Difference (NPD)

Article DOI: 10.3791/57006-v • 09:47 min • September 13th, 2018
September 13th, 2018 •
1, 2, 3, 4, 5, 1, 6,7
1Department of Medicine and the Gregory Fleming James Cystic Fibrosis Center, University of Alabama at Birmingham, 2Department of Pulmonology and Tuberculosis, University Medical Center Utrecht, 3Center for Experimental Medicine, Queens University, Northern Ireland, 4Hadassah Hebrew University Medical Center, Jerusalem, 5Centro Fibrosi Cistica, Azienda Ospedaliera Universitaria Integrata, 6Service de Pneumologie et Allergologie Pédiatriques and Center de Ressources et de Compétence de la Mucoviscidose, Hôpital Necker Enfants Malades, 7INSERM U 1151, Institut Necker Enfants Malades

Capitoli

Riepilogo
Automatic Translation

Here, we present a standardized protocol to measure the nasal potential difference (NPD). Cystic fibrosis transmembrane conductance regulator (CFTR) and epithelial sodium channel (ENaC) function are evaluated by the change in the voltage across the nasal epithelium after superfusion of solutions that modify ion channel activity, providing an outcome measure.

Tags

Standardized Measurement Nasal Membrane Transepithelial Potential Difference NPD CFTR Function Epithelial Sodium Channel Airway Epithelium Cystic Fibrosis Genetic Disorders Hyperactive Epithelial Sodium Channel Function Thao Nguyen Aur Voltage Change Ion Channel Activity Nasal Epithelium Subcutaneous Compartment Inferior Nasal Turbinate Amiloride Inhibitor
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter