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Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants
 

Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants

Article DOI: 10.3791/57081-v • 08:12 min • March 29th, 2018
March 29th, 2018 •
1,2, 1,2, 1,2, 1,2, 1,2
1Institut National de la Santé et Recherche Médicale (INSERM) UMRS 1138, Paris-Diderot University, Pierre & Marie Curie University, Paris-Descartes University, Laboratory of Molecular Oral Pathophysiology, Cordeliers Research Centre, 2Unit of Formation and Research (UFR) of Odontology, Paris-Diderot University

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Understanding enamel formation and possible alterations requires the study of ameloblast activity. Here, we describe a reliable and consistent method to micro-dissect enamel organs containing secretion- and maturation-stage ameloblasts that may be used for further quantitative and qualitative experimental procedures.

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Keywords: Enamel Organ Mandibular Incisor Rats Environmental Toxicants Dental Epithelium Enamel Synthesis Enamel Pathologies Dental Fluorosis Molar Incisor Hyperenamelization Hereditary Enamel Genesis Imperfectum Immunohistochemistry Hemogenesis Euthanasia Hemi-mandible Temporal-mandibular Joint Cervical Loop Ameloblasts PBS
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