È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 00:46Expression of His6-TIp3-LBD in E. coli
- 02:29Isolation and Denaturation of Inclusion Bodies
- 05:34Protein Refolding
- 07:39Purification of His6-tagged Protein Using Immobilized Metal Ion Affinity Chromatogrphy
- 08:49His6-tag Removal Using TEV Protease and Size-excluson Chromatography of Tlp3-LBD
- 11:06Results: Expression, Refolding, and Purification of Tlp3-LBD
- 12:41Conclusion
