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Immunology and Infection

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JoVE Journal Immunology and Infection
Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis
 

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

Article DOI: 10.3791/57155-v • 09:52 min • March 9th, 2018
March 9th, 2018 •
1, 1, 1, 1, 1
1Department of Microbiology and Immunology, Indiana University School of Medicine - Terre Haute

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Here we describe a protocol to determine pulmonary fungal burden in mice with invasive aspergillosis by quantification of Gomori's modified methanamine silver staining in histological sections. Use of this method resulted in comparable results with less animals compared to assessment of fungal burden by quantitative PCR of lung fungal DNA.

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Keywords: Histological Quantification Lung Fungal Burden Invasive Aspergillosis Experimental Aspergillosis Quantitative PCR Neutrophil Depletion Aspergillus Fumigatus Conidia Intranasal Infection Histological Analysis
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