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Developmental Biology

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JoVE Journal Developmental Biology
Fetal Mouse Cardiovascular Imaging Using a High-frequency Ultrasound (30/45MHZ) System
 

Fetal Mouse Cardiovascular Imaging Using a High-frequency Ultrasound (30/45MHZ) System

Article DOI: 10.3791/57210-v • 07:34 min • May 5th, 2018
May 5th, 2018 •
1,2
1Neonatal/Congenital Heart Laboratory, Cardiovascular Research Laboratory, David Geffen School of Medicine, University of California, Los Angeles, 2Children's Discovery and Innovation Institute, Department of Pediatrics, David Geffen School of Medicine, University of California, Los Angeles

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High-frequency ultrasound imaging of the fetal mouse has improved imaging resolution and can provide precise non-invasive characterization of cardiac development and structural defects. The protocol outlined herein is designed to perform real-time fetal mice echocardiography in vivo.

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Fetal Mouse Cardiovascular Imaging High-frequency Ultrasound 30/45MHz Structure Hemodynamic Parameters Phenotyping Cardiac Morphology Flow Indices Congenital Heart Defects Experimental Settings Sensitivity Resolution Two-dimensional Dynamic Images Ultrasound Imaging System Physiology Monitoring Unit Transducer Scan Head Cardiac Measurement Program Option Ultrasound Gel Pre-warming Container Anesthesia Tubing System Oxygen Levels Isoflurane Levels Disinfecting Imaging Platform Body Temperature Maintenance Sedated Dam Supine Position Steady State Sedation
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