Rivista
/
Ricerca sul cancro
/
复杂核型细胞周期捕获细胞的生成与分离
JoVE Journal
Ricerca sul cancro
This content is Free Access.
JoVE Journal Ricerca sul cancro
Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes
Please note that all translations are automatically generated. Click here for the English version.

复杂核型细胞周期捕获细胞的生成与分离

DOI:
10.3791/57215-v

•

05:22 min

•

April 13, 2018

•

, ,
1Koch Institute for Integrative Cancer Research at MIT, Department of Biology,Massachusetts Institute of Technology

Capitoli

  • 00:04Titolo
  • 00:32Synchronization of RPE-1 hTERT Cells
  • 01:34Generation of Aneuploid Cells by Interference with the Activity of the Mitotic Kinase Mps1 and Removal of Aneuploid Cycling Cells to Enrich the ArCK Population
  • 03:36Results: In Vitro Isolation and Characterization of Aneuploid Cells with Complex Karyotypes (ArCK)
  • 04:35Conclusion

Summary

Traduzione automatica

Traduzione automatica

体检导致基因组不稳定, 最终产生细胞周期-被捕获的细胞与复杂的核型。本文提供了一种简单方便的分离异倍体细胞的方法。

Tags

Keywords: Cell Cycle ArrestComplex KaryotypesAneuploid CellsCell SynchronizationRPE-1 HTERT CellsThymidineMps1 Inhibitor ReversineNocodazoleMitotic Shake-offArCK Cells

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/57215?article_type=v

来自沙柯玛患者衍生异种移植物的肿瘤启动细胞的分离和表征
check_url/it/57215?article_type=v

小鼠单核细胞衍生树突状细胞的分离及与肿瘤免疫配合物的后体外活化
check_url/it/57215?article_type=v

骨髓间充质干细胞在浆组织中的分离及与癌细胞联合培养研究其相互作用
check_url/it/57215?article_type=v

利用人类诱导多能干细胞生成肿瘤抗原特异性T细胞
check_url/it/57215?article_type=v

细胞毒性细胞因子诱导的杀伤性T细胞的分离和扩张，用于癌症治疗
check_url/it/57215?article_type=v

正交胰腺肿瘤的产生与肿瘤渗透T细胞细胞毒性的外体特征
check_url/it/57215?article_type=v

癌症患者血浆样本中无细胞DNA检测
check_url/it/57215?article_type=v

从细胞和组织样本中分离和功能评估人类乳腺癌干细胞
check_url/it/57215?article_type=v

鼠心肺复合物的脱细胞化
check_url/it/57215?article_type=v

组织中蛋白质和RNA表达的空间分析：一种微调虚拟显微切割的方法

Read Article