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Journal / Bioengineering / Gel-seq: A Method for Simultaneous Sequencing…
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Bioengineering

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Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices

Article DOI: 10.3791/57315-v • 09:19 min • March 26th, 2018
March 26th, 2018 •
*1, *2, 2, 3, 2
1Department of Engineering, University of San Diego, 2Department of Bioengineering, University of California, San Diego, 3Department of Mechanical and Aerospace Engineering, University of California, San Diego
* These authors contributed equally

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Gel-seq enables researchers to simultaneously prepare libraries for both DNA- and RNA-seq at negligible added cost starting from 100 - 1000 cells using a simple hydrogel device. This paper presents a detailed approach for the fabrication of the device as well as the biological protocol to generate paired libraries.

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Keywords: Gel-seq Simultaneous Sequencing DNA And RNA Hydrogel Matrices Next-generation Sequencing Genomic And Transcriptomic Information Filler Gel High Density Gel Low Density Gel Gel Electrophoresis Cassette Polymer Curing
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