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Biochemistry

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JoVE Journal Biochemistry
Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction
 

Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction

Article DOI: 10.3791/57334-v • 12:38 min • February 15th, 2018
February 15th, 2018 •
1
1Institute of Physiological Chemistry and Pathobiochemistry, University of Münster

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A detailed protocol to perform a titration ELISA is described. Moreover, a novel algorithm is presented to evaluate titration ELISAs and to obtain a dissociation constant of binding of a soluble ligand to a microtiter plate-immobilized receptor.

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Keywords: Titration ELISA Dissociation Constant Receptor-ligand Interaction Integrin Alpha 2 A Domain Rhodocetin Affinity Constant Protein-ligand Interaction Receptor Research Pharmaceutical Research Pharmacology
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