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Rapid In Vivo Assessment of Adjuvant’s Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development
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JoVE Journal Medicina
Rapid In Vivo Assessment of Adjuvant’s Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development

Rapid In Vivo Assessment of Adjuvant’s Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development

DOI:
10.3791/57401-v

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09:03 min

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June 19, 2018

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, , , , , , ,
1Department of Vaccinology and Applied Microbiology,Helmholtz Centre for Infection Research

Capitoli

  • 00:05Titolo
  • 01:03OT-I T Cell Staining and Adoptive Transfer
  • 04:22Endo-Free OVA +/- Adjuvant Immunization and Lymphocyte Isolation
  • 05:21Flow Cytometry
  • 07:30Results: Representative Adjuvant Drive Cytotoxic T Cell Generation
  • 08:31Conclusion

Summary

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We present here an application for a standard immunological technique (CFSE stained OT-I proliferation) intended to rapidly monitor adjuvant-mediated cytotoxic T lymphocyte (CTL) generation in vivo. This fast estimation of CTL capacities is useful for the development of prophylactic vaccines against intracellular pathogens as well as therapeutic cancer vaccines.

Tags

OT1 CD8 T CellsCFSEAdoptive TransferAdjuvantCytotoxic T Lymphocyte (CTL) ResponseVaccine DevelopmentLymph NodeSpleenMagnetic Bead Isolation

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