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Bioengineering

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JoVE Journal Bioengineering
Assessing Collagen and Elastin Pressure-dependent Microarchitectures in Live, Human Resistance Arteries by Label-free Fluorescence Microscopy
 

Assessing Collagen and Elastin Pressure-dependent Microarchitectures in Live, Human Resistance Arteries by Label-free Fluorescence Microscopy

Article DOI: 10.3791/57451-v • 09:58 min • April 9th, 2018
April 9th, 2018 •
1, 2, 2, 1,3
1Department of Cardiovascular and Renal Research, Institute of Molecular Medicine, University of Southern Denmark, 2Department of Biochemistry and Molecular Biology, University of Southern Denmark, 3Department of Cardiac, Thoracic and Vascular Surgery, Odense University Hospital

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We describe simultaneous mechanical testing and 3D-imaging of the arterial wall of isolated, live human resistance arteries, and Fiji and Ilastik image analyses for the quantification of elastin and collagen spatial organization and volume densities. We discuss the use of these data in mathematical models of arterial wall mechanics.

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Keywords: Collagen Elastin Microarchitecture Resistance Arteries Label-free Fluorescence Microscopy Two-photon Microscopy Extracellular Matrix Vascular Mechanics Artery Pressurization Second Harmonic Generation Autofluorescence Fiji Image Analysis
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