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Immunology and Infection

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JoVE Journal Immunology and Infection
Assembly and Purification of Prototype Foamy Virus Intasomes
 

Assembly and Purification of Prototype Foamy Virus Intasomes

Article DOI: 10.3791/57453-v • 10:20 min • March 19th, 2018
March 19th, 2018 •
1, 1, 1
1Department of Cancer Biology and Genetics, The Ohio State University College of Medicine

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Recombinant retroviral integrase and DNA oligomers mimicking viral DNA ends can form an enzymatically active complex known as an intasome. Intasomes may be used for biochemical, structural, and kinetic studies. This protocol details how to assemble and purify prototype foamy virus intasomes.

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Keywords: Foamy Virus Intasomes Integration Complexes Viral DNA PFV Integrase Dialysis Annealing Ultracentrifugal Filtration Buffer Exchange UV Absorbance
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