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Making, Testing, and Using Potassium Ion Selective Microelectrodes in Tissue Slices of Adult Brain
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JoVE Journal Neuroscienze
Making, Testing, and Using Potassium Ion Selective Microelectrodes in Tissue Slices of Adult Brain

Making, Testing, and Using Potassium Ion Selective Microelectrodes in Tissue Slices of Adult Brain

DOI:
10.3791/57511-v

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11:20 min

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May 07, 2018

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, , ,
1Department of Physiology, David Geffen School of Medicine,University of California Los Angeles, 2Department of Neurology, David Geffen School of Medicine,University of California Los Angeles, 3Department of Neurobiology, David Geffen School of Medicine,University of California Los Angeles

Capitoli

  • 00:14Titolo
  • 00:46Preparation of Potassium Ion-selective Microelectrodes
  • 04:18Calibration of Potassium Ion-selective Microelectrodes
  • 05:40Acute Slice Preparation
  • 07:16Measurement of Electrically Evoked K Release
  • 09:26Results: Calibration of Microelectrodes and Measurement of Electrically Evoked K Release
  • 10:53Conclusion

Summary

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Potassium ions contribute to the resting membrane potential of cells and extracellular K+ concentration is a crucial regulator of cellular excitability. We describe how to make, calibrate and use monopolar K+-selective microelectrodes. Using such electrodes enables the measurement of electrically evoked K+ concentration dynamics in adult hippocampal slices.

Tags

Potassium Ion Selective MicroelectrodesAdult BrainPotassium Ion HomeostasisBorosilicate Glass CapillariesSilanizationMicroelectrode FabricationDDS Silanization SolutionMicroelectrode PullerPotassium Ion Concentration Measurement

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