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Medicine
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均質な時間分解蛍光共鳴エネルギー転送を用いた測定インスリン分泌の検出
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JoVE Journal
Medicine
Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion
Please note that all translations are automatically generated.
Click here for the English version.
均質な時間分解蛍光共鳴エネルギー転送を用いた測定インスリン分泌の検出
DOI:
10.3791/57531-v
•
07:30 min
•
May 10, 2018
•
Despoina Aslanoglou
,
Emily W. George
,
Zachary Freyberg
2
1
Department of Psychiatry
,
University of Pittsburgh
,
2
Department of Cell Biology
,
University of Pittsburgh
Chapters
00:05
Title
01:22
INS-1E Cells: Maintenance and Plating
03:04
Insulin Secretion Assay
04:12
HTRF To Measure Insulin Secretion and Data Analysis
05:17
Results: Use of HTRF Assay for the Rapid Detection of Insulin Secretion from the Cells
06:43
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここでは、提案する均質な時分割フレット (HTRF) 細胞から分泌されるインスリンの急速な検出のための効率的な方法として。
Tags
Insulin Secretion
Time-resolved FRET
Beta Cells
Glucose Stimulation
Diabetes
Drug Discovery
INS-1E Cells
Cell Culture
Trypsin
Cell Counting
Viability
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