Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / Studying the Hypothalamic Insulin Signal to…
JoVE Journal
Medicine

This content is Open Access.

JoVE Journal Medicine
Studying the Hypothalamic Insulin Signal to Peripheral Glucose Intolerance with a Continuous Drug Infusion System into the Mouse Brain
 

Studying the Hypothalamic Insulin Signal to Peripheral Glucose Intolerance with a Continuous Drug Infusion System into the Mouse Brain

Article DOI: 10.3791/56410-v • 08:32 min • January 4th, 2018
January 4th, 2018 •
1,2, 1,2,3
1The Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University and National Health Research, 2Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University, 3TMU research center for Neurotrauma and Neuroregeneration, College of Medical Science and Technology, Taipei Medical University

Chapters

Summary
Automatic Translation

This protocol studies the role of chemokine (C-C motif) ligand 5 (CCL5) in the hypothalamus by delivering an antagonist, MetCCL5, into the mouse brain using a micro-osmotic pump brain infusion system. This transient inhibition of CCL5 activity interrupted hypothalamic insulin signaling, leading to glucose intolerance and peripheral systemic insulin sensitivity.

Tags

Keywords: Hypothalamic Insulin Signal Peripheral Glucose Intolerance C-C-L-5 Signaling Met-C-C-L-5 Micro-osmotic Pump Artificial Cerebrospinal Fluid Brain Infusion Kit Stereotactic Apparatus Anesthetized Mouse Brain Map
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter