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Neuroscience

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JoVE Journal Neuroscience
Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy
 

Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy

Article DOI: 10.3791/57546-v • 08:56 min • May 11th, 2018
May 11th, 2018 •
*1,2, *2,3, 1,2
1Molecular and Cell Biology, Taiwan International Graduate Program, Academia Sinica and Graduate Institute of Life Sciences, National Defense Medical Center, 2Institute of Molecular Biology, Academia Sinica, 3Institute of Biotechnology, College of Bio-Resources and Agriculture, National Taiwan University
* These authors contributed equally

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Here, we describe a protocol for visualizing motor neuron projection and axon arborization in transgenic Hb9::GFP mouse embryos. After immunostaining for motor neurons, we used light sheet fluorescence microscopy to image embryos for subsequent quantitative analysis. This protocol is applicable to other neuron navigation processes in the central nervous system.

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Keywords: Motor Axon Axon Arborization Light Sheet Fluorescence Microscopy Mouse Embryos Motor Neuron Development GFP Antibody Staining Tissue Clearing Image Analysis
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