A11-positive &#946;-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis

Huang Chunhui; Xu Dilin; Zhang Ke; Shentu Jieyi; Yan Sicheng; Wu Dapeng; Wang Qinwen; Cui Wei

doi:10.3791/57592

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Biochimica

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A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis

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Biochimica

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JoVE Journal Biochimica

A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis

DOI:

10.3791/57592-v

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06:17 min

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May 22, 2018

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Huang Chunhui², Xu Dilin, Zhang Ke, Shentu Jieyi, Yan Sicheng, Wu Dapeng, Wang Qinwen, Cui Wei²

¹Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine,Ningbo University, ²Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center,Ningbo University, ³School of Materials Science and Chemical Engineering,Ningbo University

Capitoli

00:05Titolo
00:31Sample Preparation
02:08Dot Blotting Analysis
03:47Results: Use of Synthetic Peptides and Dot Blot Analysis to Prepare and Evaluate the Amount of Aβ Oligomers
05:40Conclusion

Summary

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This protocol describes how to prepare Aβ oligomers from a synthetic peptide in vitro and to evaluate relative amounts of Aβ oligomer by a dot blotting analysis.

A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis

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