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A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis
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Biochemistry
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JoVE Journal Biochemistry
A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis

A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis

DOI:
10.3791/57592-v

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06:17 min

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May 22, 2018

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, , , , , , ,
1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine,Ningbo University, 2Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center,Ningbo University, 3School of Materials Science and Chemical Engineering,Ningbo University

Chapters

  • 00:05Title
  • 00:31Sample Preparation
  • 02:08Dot Blotting Analysis
  • 03:47Results: Use of Synthetic Peptides and Dot Blot Analysis to Prepare and Evaluate the Amount of Aβ Oligomers
  • 05:40Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes how to prepare Aβ oligomers from a synthetic peptide in vitro and to evaluate relative amounts of Aβ oligomer by a dot blotting analysis.

Tags

Keywords: A-beta OligomerDot Blotting AnalysisCurcuminAnti-oligomer Antibody A11Anti-A-beta Antibody 6E10Nitrocellulose MembraneBovine Serum AlbuminTBST BufferMagnetic AgitatorCentrifugation

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