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Biochemistry

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JoVE Journal Biochemistry
Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
 

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Article DOI: 10.3791/57674-v • 12:26 min • May 3rd, 2018
May 3rd, 2018 •
*1, *1, 1, 1
1Department of Chemistry, State University of New York at Binghamton
* These authors contributed equally

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Cyclin-dependent kinase 1 (Cdk1) is activated in the G2 phase of the cell cycle and regulates many cellular pathways. Here, we present a protocol for an in vitro kinase assay with Cdk1, which allows the identification of Cdk1-specific phosphorylation sites for establishing cellular targets of this important kinase.

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Keywords: CDK1 Phosphorylation Kinase Assay Cell Cycle CENP-F Protein Expression E. Coli LB Medium Antibiotics Protein Purification
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