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JoVE Journal
Developmental Biology

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Preparing Developing Peripheral Olfactory Tissue for Molecular and Immunohistochemical Analysis in Drosophila
 

Preparing Developing Peripheral Olfactory Tissue for Molecular and Immunohistochemical Analysis in Drosophila

Article DOI: 10.3791/57716-v 09:18 min June 13th, 2018
June 13th, 2018

Capitoli

Riepilogo

Here, we present a protocol to stage and dissect developing olfactory tissue from Drosophila species. The dissected tissue can later be used for molecular analyses, such as quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction) or RNA sequencing (RNAseq), as well as in vivo analyses such as immunohistochemistry or in situ hybridization.

Tags

Keywords: Olfactory Tissue Drosophila Molecular Analysis Immunohistochemistry Peripheral Olfactory System Antennal Disc RNA Sequencing Prepupal Stage Pupal Development Dissection RNA Isolation
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