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间歇酵母 2-混合筛序列数据的信息化分析
JoVE Journal
Genetica
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JoVE Journal Genetica
Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens
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间歇酵母 2-混合筛序列数据的信息化分析

DOI:
10.3791/57802-v

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09:14 min

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June 28, 2018

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, , ,
1Molecular Physiology and Biophysics,University of Iowa

Capitoli

  • 00:04Titolo
  • 00:50Mapping Fastq Files
  • 02:14Bioinformatics Processing Using DEEPN Software
  • 06:08Verification of Candidates Identified by DEEPN
  • 08:01Conclusion

Summary

Traduzione automatica

Traduzione automatica

酵母菌群的深度测序为阳性酵母 2-混合相互作用可能产生大量的信息关于相互作用的伙伴蛋白。在这里, 我们描述了具体的生物信息学工具的操作和定制的更新软件来分析这些屏幕的序列数据。

Tags

Keywords: Informatic AnalysisSequence DataBatch Yeast 2-hybrid ScreensProtein InteractomesBioinformatics ProcessingUser-friendly Graphic User InterfaceMolecular BiologistsCell BiologistsBiochemistsMAPster SoftwareDEEPN AnalysisHISAT2Multi-threadingReference DNA GenomeY2H Prey Library InsertsMapped FilesUnmapped Reads

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