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快速、安全、简便的人工床边核酸提取法检测全血标本中的病毒
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Rapid, Safe, and Simple Manual Bedside Nucleic Acid Extraction for the Detection of Virus in Whole Blood Samples
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快速、安全、简便的人工床边核酸提取法检测全血标本中的病毒

DOI:
10.3791/58001-v

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06:59 min

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June 30, 2018

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, ,
1Virus Research & Development Laboratory,Statens Serum Institut, 2Infectious Disease Research Unit,University of Southern Denmark

Capitoli

  • 00:04Titolo
  • 00:44Preparation for Virus Inactivation and Rapid Nucleic Acid Extraction
  • 02:02Blood Collection from Patients with Signs and Symptoms of a Virus Infection
  • 02:50Simplified Nucleic Acid Extraction from Whole Blood
  • 05:04Results: Sensitivity of the Simplified NA Extraction Method
  • 06:22Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里, 我们提出了一个快速病毒核酸提取从病毒灭活全血的协议。萃取是直接在采血管, 不需要设备或电力。该方法不依赖于实验室设施, 可以在任何地方使用 (例如在野战医院)。

Tags

Manual Bedside Nucleic Acid ExtractionVirus DetectionWhole Blood SamplesPoint-of-care DiagnosticsBlood CollectionMagnetic Glass ParticlesWash BuffersElution BufferVirus InactivationNucleic Acid Purification

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