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Journal / Biochemistry / Covalent Immobilization of Proteins for the…
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Biochemistry

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JoVE Journal Biochemistry
Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
 

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

Article DOI: 10.3791/58167-v • 11:13 min • August 20th, 2018
August 20th, 2018 •
1,2,3, 2, 1,3, 3, 2, *4, *1,3
1Center for Applied Tissue Engineering and Regenerative Medicine, Munich University of Applied Sciences, 2FG Protein Biochemistry & Cellular Microbiology, Munich University of Applied Sciences, 3Center for Nano Science, Ludwig-Maximilians-Universität München, 4Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie, University Medical Center Göttingen
* These authors contributed equally

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This protocol describes the covalent immobilization of proteins with a heterobifunctional silane coupling agent to silicon-oxide surfaces designed for the atomic force microscopy based single molecule force spectroscopy which is exemplified by the interaction of RrgA (pilus-1 tip adhesin of S. pneumoniae) with fibronectin.

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Covalent Immobilization Proteins Single Molecule Force Spectroscopy Biophysical Field Protein Interactions Biomolecules Atomic Force Microscope Cantilever Probe Acid Resistant Gloves Safety Goggles Laboratory Coat Isopropanol Lint-free Precision Wipes Glass Slides Hydrochloric Acid Ultrasonic Bath Silicon Nitride AFM Cantilever Probes UV Light-impermeable Chamber Ultraviolet Light Staining Jar Doubly Distilled Water Ethoxy Silane Polyethylene Glycol Acid
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