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烟草 Benthamiana叶在制备鳞片上的瞬态表达
JoVE Journal
Biochimica
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JoVE Journal Biochimica
Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
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烟草 Benthamiana叶在制备鳞片上的瞬态表达

DOI:
10.3791/58169-v

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08:56 min

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August 16, 2018

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, , ,
1John Innes Centre

Capitoli

  • 00:04Titolo
  • 00:33Prepare Infiltration Suspension
  • 02:11Perform Vacuum Infiltration
  • 03:56Growth and Harvest of the Infiltrated Leaves and Pressurized Solvent Extraction
  • 05:38Basic Ion Exchange Resin Treatment for Removal of Chlorophylls
  • 06:58Results: Infiltration Coverage and Isolated Triterpene Product
  • 07:49Conclusion

Summary

Traduzione automatica

Traduzione automatica

烟草 benthamiana叶片中生物合成酶的瞬态异源表达可以将 23-oxidosqualene 的内源供给转移到新的高附加值三萜产品的生产中。这里描述了一个详细的协议, 快速 (5 天) 准备规模生产萜和类似物利用这个强大的植物基础平台。

Tags

Transient ExpressionNicotiana BenthamianaTriterpene ProductionPreparative ScaleAgrobacterium TumefaciensCo-infiltrationVacuum InfiltrationMMA BufferOD 600Preparative-scale AccessStructural CharacterizationMedicinally Relevant Biological ActivitySelective LB AgarSelective LB MediaCentrifugationResuspensionInfiltration Suspension

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