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Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism
 

Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism

Article DOI: 10.3791/58192-v • 07:39 min • September 30th, 2018
September 30th, 2018 •
1, 2, 1, 3
1Department of Chemistry, Universidad Autónoma de Querétaro, 2Department of Biochemical Engineering, Instituto Tecnológico de Morelia, 3Department of Biochemical Engineering, Instituto Tecnológico Superior de Ciudad Hidalgo

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Here we present a protocol to estimate the respiratory and fermentative metabolism by fitting the exponential growth of Saccharomyces cerevisiae to the exponential growth equation. Calculation of the kinetic parameters allows for the screening of influences of substances/compounds on fermentation or mitochondrial respiration.

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Keywords: Saccharomyces Cerevisiae Exponential Growth Kinetics Batch Culture Respiratory Metabolism Fermentative Metabolism Warburg Effect Crabtree Effect Yeast Extract Peptone Dextrose (YPD) Broth Glycerol Preservation Agar Plate Microplate Growth Curve Shaken Conical Flask Optical Density Agitation Speed
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