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Real Time In Vivo Tracking af thymocytter i det forreste kammer i øjet ved Laser Scanning mikroskopi
 
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Real Time In Vivo Tracking af thymocytter i det forreste kammer i øjet ved Laser Scanning mikroskopi

Article DOI: 10.3791/58236-v • 08:21 min • October 2nd, 2018
October 2nd, 2018 •
1,2, 3
1School of Medicine and Dentistry, Universidad Católica de Valencia San Vicente Mártir, 2Unidad Mixta CIPF-UCV, Centro de Investigación Príncipe Felipe, 3Department of Medicine, University of Miami

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Målet med protokollen er at vise langsgående intravital real-time tracking af thymocytter ved laser scanning mikroskopi i thymic implantater i mus øjet forkammeret. Gennemsigtigheden af hornhinden og vascularization af graften giver mulighed for uafbrudt optagelse stamfader celle rekruttering og modne T-celle udgang.

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Biologi spørgsmål 140 Thymus graft øjet forkammeret migration thymocyte stamfader cortex medulla konfokalmikroskopi
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