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5-甲基胞嘧啶和 5-Hydroxymethylcytosine 在小鼠视网膜发育和分裂后中的免疫组化检测
JoVE Journal
Biologia dello sviluppo
This content is Free Access.
JoVE Journal Biologia dello sviluppo
Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina
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5-甲基胞嘧啶和 5-Hydroxymethylcytosine 在小鼠视网膜发育和分裂后中的免疫组化检测

DOI:
10.3791/58274-v

•

07:50 min

•

August 29, 2018

•

, , ,
1BioTime, Inc

Capitoli

  • 00:04Titolo
  • 00:59Tissue Preparation for Immunostaining
  • 03:02Immunostaining with 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) Antibodies
  • 05:02Results: Immunostaining with 5mC and 5hmC to Determine Their Distribution During Retinal Development
  • 06:49Conclusion

Summary

Traduzione automatica

Traduzione automatica

本报告的目的是描述在开发和分裂后小鼠视网膜时, 对表观遗传标记、5-甲基胞嘧啶 (5mC) 和 5-hydroxymethylcytosine (5hmC) 的鲁棒免疫组化检测的协议。

Tags

Keywords: Immunohistochemistry5-methylcytosine5-hydroxymethylcytosineMouse RetinaRetinal DevelopmentChromatinFrozen Histological PreparationCryosectioningImmunostainingDNA Methylation

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