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JoVE Journal
Immunology and Infection

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Oral Intubation of Adult Zebrafish
 

Oral Intubation of Adult Zebrafish: A Model for Evaluating Intestinal Uptake of Bioactive Compounds

Article DOI: 10.3791/58366-v 04:33 min September 27th, 2018
September 27th, 2018

Capitoli

Riepilogo

The protocol describes intubating adult zebrafish with a biologic; then dissecting and preparing the intestine for cytometry, confocal microscopy and qPCR. This method allows administration of bioactive compounds to monitor intestinal uptake and the local immune stimulus evoked. It is relevant for testing the intestinal dynamics of oral prophylactics.

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Keywords: Oral Intubation Zebrafish Intestinal Uptake Bioactive Compounds Mucosa Vaccinology Nanoparticle Suspension Intestinal Dissection Anesthetizing Recovery Tank Euthanizing
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