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Cancer Research

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JoVE Journal Cancer Research
Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens
 

Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens

Article DOI: 10.3791/58390-v • 10:49 min • January 21st, 2019
January 21st, 2019 •
1, 1, 1, 1, 2, 1, 3, 3, 1, 2, 1, 1, 4, 3, 1
1Laboratory of Pathology, MedImmune, 2Laboratory of Pathology, MedImmune, 3Akoya Biosciences Inc., 4Department of Translational Molecular Pathology, University of Texas - MD Anderson Cancer Center

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Automatic Translation

A detailed protocol for a six-marker multiplex immunofluorescence panel is optimized and performed, using an automated stainer for more consistent results and a shorter procedure time. This approach can be directly adapted by any laboratory for immuno-oncology studies.

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Keywords: Multiplex Immunofluorescence Immuno-oncology Formalin-fixed Carcinoma Tissue Automated Staining Protocol Optimization Immune Cell Phenotyping Prognostic And Predictive Biomarkers Spectral Imaging Peroxidase Inhibition Primary And Secondary Antibodies Tyramide Signal Amplification Antigen Retrieval Drop Controls Isotype Controls
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