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Immunologia e infezioni

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成人斑马鱼对 DNA 疫苗临床前筛选的免疫研究

JoVE Journal
Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Immunization of Adult Zebrafish for the Preclinical Screening of DNA-based Vaccines

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成人斑马鱼对 DNA 疫苗临床前筛选的免疫研究

DOI:

10.3791/58453-v

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05:39 min

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October 30, 2018

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Henna Myllymäki*¹, Mirja Niskanen*¹, Kaisa Oksanen², Mika Rämet^3,4,5

¹BioMediTech Institute, Faculty of Medicine and Life Sciences,University of Tampere, ²MedEngine Oy, ³Department of Pediatrics,Tampere University Hospital, ⁴Department of Children and Adolescents,Oulu University Hospital, ⁵PEDEGO Research Unit, Medical Research Center,University of Oulu

Capitoli

00:04Titolo
00:35Pulling the Microinjection Needles
01:10Filling the Micropipette Needles
01:49Setting the Microinjector and Electroporator for Immunization
02:40Injection of the DNA Vaccine and Electroporation and Visualization of Antigen Expression
04:04Results: Danio rerio Injected with DNA-based Vaccine
05:04Conclusion

Summary

Traduzione automatica

在这里, 我们描述了一项关于成人斑马鱼 (斑马斑马) 免疫接种的协议, 以 DNA 为基础的疫苗, 并证明了成功的疫苗接种事件的验证。该方法适用于各种感染模型的疫苗候选者的临床前筛选。

Tags

Adult Zebrafish DNA-based Vaccines Preclinical Screening Tuberculosis Cost-effective Injection Plasmid Micro Manipulator Electroporator Anesthesia

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