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Microdialysis of Excitatory Amino Acids During EEG Recordings in Freely Moving Rats
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Neuroscienze
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JoVE Journal Neuroscienze
Microdialysis of Excitatory Amino Acids During EEG Recordings in Freely Moving Rats

Microdialysis of Excitatory Amino Acids During EEG Recordings in Freely Moving Rats

DOI:
10.3791/58455-v

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08:47 min

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November 08, 2018

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, , , , , ,
1Department of Medical Sciences, Section of Pharmacology, Neuroscience Center,University of Ferrara and National Institute of Neuroscience

Capitoli

  • 00:04Titolo
  • 00:42Epileptic Behavior Monitoring
  • 01:52Microdialysis Preparation
  • 03:54Microdialysis: Collection of Samples
  • 06:46Results: Extracellular Concentrations of Glutamate are Higher in Chronic Epileptic Rats
  • 07:34Conclusion

Summary

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Here, we describe a method for in vivo microdialysis to analyze aspartate and glutamate release in the ventral hippocampus of epileptic and non-epileptic rats, in combination with EEG recordings. Extracellular concentrations of aspartate and glutamate may be correlated with the different phases of the disease.

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Keywords: MicrodialysisExcitatory Amino AcidsEEGFreely Moving RatsNeurotransmitter ReleaseNeurological DiseasesFaraday CageEEG AmplifierEEG SoftwareEEG SignalTethered EEG Recording SystemAmplification FactorRinger’s SolutionFEP TubingGuide CannulaPlexiglass Cylinder

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