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Gene Expression Analysis of Endothelial Cells Exposed to Shear Stress Using Multiple Parallel-plate Flow Chambers
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Capitoli
- 00:04Titolo
- 00:55Assay Setup
- 04:20Flow Assay
- 05:18Cell Harvest and RNA Extraction
- 06:48Results: Representative KLF2 Loss-of-Function Analysis in Human Endothelial Cells in Response to Shear Stress
- 08:06Conclusion
Here, a workflow for the culture and gene expression analysis of endothelial cells under fluid shear stress is presented. Included is a physical arrangement for simultaneously housing and monitoring multiple flow chambers in a controlled environment and the use of an exogenous reference RNA for quantitative PCR.
