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用于玻璃化和变暖步骤中囊胚行为客观评估的形态学协议
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JoVE Journal Bioingegneria
Morphometric Protocol for the Objective Assessment of Blastocyst Behavior During Vitrification and Warming Steps
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用于玻璃化和变暖步骤中囊胚行为客观评估的形态学协议

DOI:
10.3791/58540-v

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08:28 min

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February 28, 2019

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1Department of Reproductive Medicine and Gynecological Endocrinology,University Medical Centre Maribor

Capitoli

  • 00:04Titolo
  • 00:41Blastocyst Recording During Equilibration, Cross-Sectional Area Measurement, Data Editing and Creation of a Line Diagram
  • 03:27Blastocyst Time-Lapse Re-Expansion Recording, Measurements, Data Editing and Creation of Line Diagram
  • 06:09Results: Representative Blastocoel Contraction and Re-Expansion Analyses
  • 07:49Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里, 我们提出了一个延时形态测量方案, 以跟踪在预防干预和升温后恢复过程中囊胚收缩和再膨胀的强度。该方案可在配备延时显微镜的体外受精实验室中应用, 并建议在开发最佳囊胚玻璃化方法时使用。

Tags

BlastocystMorphometricVitrificationWarmingShrinkageRe-expansionTime-lapse MicroscopyZona PellucidaTrophectodermInner Cell MassEquilibration MediumLaser PulseMicrodropletCross-sectional AreaIn Vitro Fertilization

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