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Plant Growth and Agrobacterium-mediated Floral-dip Transformation of the Extremophyte Schrenkiella parvula
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Genetica
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JoVE Journal Genetica
Plant Growth and Agrobacterium-mediated Floral-dip Transformation of the Extremophyte Schrenkiella parvula

Plant Growth and Agrobacterium-mediated Floral-dip Transformation of the Extremophyte Schrenkiella parvula

DOI:
10.3791/58544-v

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06:32 min

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January 07, 2019

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, , , ,
1Department of Biological Sciences,Louisiana State University

Capitoli

  • 00:04Titolo
  • 01:19Planting S. parvula Plants and Argobacterium-mediated Transformation of S. parvula
  • 02:38Post-transformation Plant Care and the Second Transformation
  • 03:32Selection of Positive Transformants
  • 04:46Results: Transgenic S. parvula Plants Transformed with 0.033% Efficiency
  • 05:21Conclusion

Summary

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Agrobacterium-mediated transformation using a floral-dip method can be successfully employed to create stable transgenic lines of the extremophyte model Schrenkiella parvula. We present a protocol modified from that for Arabidopsis thaliana, considering different growth habits and physiological characteristics of the extremophyte.

Tags

Schrenkiella ParvulaArabidopsis ThalianaPlant TransformationAgrobacterium-mediated TransformationFloral DipExtremophytePlant AdaptationComparative GenomicsGene RegulationPlant GrowthSeed StratificationPlant SelectionInflorescenceSilique RemovalAgrobacterium Infiltration Solution

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