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重组腺相关病毒在成年小鼠中的直接注射

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Direct Intrathecal Injection of Recombinant Adeno-associated Viruses in Adult Mice

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重组腺相关病毒在成年小鼠中的直接注射

DOI:

10.3791/58565-v

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08:17 min

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February 15, 2019

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Dongxiao Li*¹, Yundu Li*², Yunyun Tian, Zuoshang Xu, Yansu Guo⁴

¹Department of Neurology,Second Hospital of Hebei Medical University, ²No.2 Middle School of Shijiazhuang, ³Department of Biochemistry and Molecular Pharmacology,University of Massachusetts Medical School, ⁴Key Laboratory of Hebei Neurology

Capitoli

00:04Titolo
00:50Mouse Preparation and Direct Intrathecal Adeno-associated Virus (AAV) Delivery
03:16Immunohistochemical Tissue Preparation
04:46Immunohistochemistry
06:27Results: Representative AAV Transduction After Direct Intrathecal Delivery
07:52Conclusion

Summary

Traduzione automatica

在这里, 我们提出了一个直接鞘内注射技术使用1% 利多因盐酸在病毒溶液中, 以确保有效的腺病毒传递给小动物, 并建立评分系统, 以预测在中央的传导效率神经系统根据利多卡因引起的短暂虚弱程度。

Tags

Keywords: Direct Intrathecal Injection Recombinant Adeno-associated Viruses Adult Mice Central Nervous System Diseases ALS 27 Gauge Needle Hamilton Syringe Genome Copies Prone Position Biosafety Hood Pelvic Girdle L5-L6 Intervertebral Space Midline Of The Spine 30-degree Angle Intradural Space

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