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Biochemistry

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JoVE Journal Biochemistry
Vastleggen en identificatie van RNA-bindende proteïnen met behulp van klik chemie-bijgewoonde RNA-interactome vangen (CARIC) strategie
 
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Vastleggen en identificatie van RNA-bindende proteïnen met behulp van klik chemie-bijgewoonde RNA-interactome vangen (CARIC) strategie

Article DOI: 10.3791/58580-v • 09:36 min • October 19th, 2018
October 19th, 2018 •
*1,2, *1,2, 1,3, 1,2,3,4,5
1College of Chemistry and Molecular Engineering, Peking University, 2Beijing National Laboratory for Molecular Sciences, Peking University, 3Peking-Tsinghua Center for Life Sciences, Peking University, 4Synthetic and Functional Biomolecules Center, Peking University, 5Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University
* These authors contributed equally

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Een gedetailleerd protocol voor de toepassing van de klik chemie-bijgewoonde RNA-interactome vastleggen (CARIC) strategie ter identificatie van eiwitten bindende naar beide taalcode-instellingen en noncoding RNAs wordt gepresenteerd.

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Biochemie kwestie 140 RNA RNA-eiwit interacties proteomics bioorthogonal chemie noncoding RNA
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