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基于滴瓣的成年哺乳动物组织单细胞转录瘤
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
DOI:

10:12 min

January 10, 2019

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Capitoli

  • 00:04Titolo
  • 00:56Dissociating Tissue (Day 1)
  • 03:46Isolating Viable and Healthy Cells (Day 1)
  • 04:46GEM (Gel Bead in Emulsion) Generation and Barcoding (Day 1)
  • 06:17NCBI’s GEO and SRA Submissions
  • 07:34Results: Bioinformatics Analysis of Single Cell Transcriptomics
  • 09:35Conclusion

Summary

Traduzione automatica

该协议描述了为基于液滴、高通量的单细胞 rna-seq 制剂准备健康的成年哺乳动物单细胞所需的一般过程和质量控制检查。还提供了测序参数、读取对齐和下游单细胞生物信息学分析。

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