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Quantitative Immunoblotting of Cell Lines as a Standard to Validate Immunofluorescence for Quantifying Biomarker Proteins in Routine Tissue Samples
 

Quantitative Immunoblotting of Cell Lines as a Standard to Validate Immunofluorescence for Quantifying Biomarker Proteins in Routine Tissue Samples

Article DOI: 10.3791/58735-v • 09:58 min • January 7th, 2019
January 7th, 2019 •
1,2, 3, 3, 1,2
1Department of Pathology and Molecular Medicine, Queen's University, 2Division of Cancer Biology and Genetics, Queen's Cancer Research Institute, 3Queen's Laboratory for Molecular Pathology, Department of Pathology and Molecular Medicine, Queen's University

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We describe the use of quantitative immunoblotting to validate immunofluorescence histology coupled with image analysis as a means of quantifying a protein of interest in formalin-fixed, paraffin-embedded (FFPE) tissue samples. Our results demonstrate the utility of immunofluorescence histology for ascertaining the relative quantity of biomarker proteins in routine biopsy samples.

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Quantitative Immunoblotting Cell Lines Immunofluorescence Biomarker Proteins Routine Tissue Samples Cancer Pathology Conventional Immunohistochemistry Specific Cell Types Biomarker Proteins In Cancer Biopsy Specimens Prediction Of Response To Cancer Treatments Protein Quantification Immortalized Cell Lines Validation Of Immunofluorescence-based Assay Multiplexed Approach Primary Biopsy Samples Lee Boudreau Shakeel Virk Queen's Laboratory For Molecular Pathology Centrifuge
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