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Une approche impartiale de l’échantillonnage des Sections TEM en neurosciences
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1Department of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center,Medical University of Graz, 2Department of Pharmacology, Otto Loewi Research Center,Medical University of Graz, 3Division of General Neurology, Department of Neurology,Medical University of Graz, 4Institute of Pathology,Medical University of Graz, 5Department of Pathology, Medical Faculty,Otto von Guericke University Magdeburg
Capitoli
- 00:04Titolo
- 01:02TEM Preparation Embedding, Ultra-Thin Sectioning and Staining
- 02:05Imaging of Corresponding ROIs on the Reference and Look-Up Sections on TEM with Software Packages
- 05:25Analyze the TEM images with ImageJ to Document Ultrastructural Features
- 07:10Using SerialEM Script to Optimize Elemental Analysis in Brain Samples in Combination with DigitalMicrograph
- 09:19Results: Unbiased Approach of Sampling TEM Sections
- 10:25Conclusion
Nous introduisons un roman "workflow" pour microscopie électronique enquêtes du tissu cérébral. La méthode permet à l’utilisateur d’examiner les fonctions neuronales de façon impartiale. Pour l’analyse élémentaire, nous présentons également un script qui automatizes la majeure partie du flux de travail pour l’échantillonnage aléatoire.
