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1Department of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center,Medical University of Graz, 2Department of Pharmacology, Otto Loewi Research Center,Medical University of Graz, 3Division of General Neurology, Department of Neurology,Medical University of Graz, 4Institute of Pathology,Medical University of Graz, 5Department of Pathology, Medical Faculty,Otto von Guericke University Magdeburg
Capitoli
- 00:04Titolo
- 01:02TEM Preparation Embedding, Ultra-Thin Sectioning and Staining
- 02:05Imaging of Corresponding ROIs on the Reference and Look-Up Sections on TEM with Software Packages
- 05:25Analyze the TEM images with ImageJ to Document Ultrastructural Features
- 07:10Using SerialEM Script to Optimize Elemental Analysis in Brain Samples in Combination with DigitalMicrograph
- 09:19Results: Unbiased Approach of Sampling TEM Sections
- 10:25Conclusion
Se introduce un nuevo flujo de trabajo para investigaciones de microscopia electrónica del tejido de cerebro. El método permite al usuario examinar características neuronales de una manera imparcial. Para el análisis elemental, también presentamos un script que automatiza la mayor parte del flujo de trabajo para el muestreo al azar.
