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原子力显微镜成像在核细胞结构和动力学研究中的应用
JoVE Journal
Biochimica
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JoVE Journal Biochimica
Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging
DOI:

09:52 min

January 31, 2019

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Capitoli

  • 00:04Titolo
  • 01:06Functionalization of Mica Surface for Static AFM Imaging of Nucleosomes
  • 02:37Preparation of Nucleosome Samples on APS-Mica for Static AFM Imaging
  • 03:39Static AFM Imaging of Nucleosomes
  • 04:42High-Speed Time-Lapse AFM Imaging of Nucleosome Dynamics
  • 07:42Results: Probing the Structure and Dynamics of Nucleosomes
  • 09:29Conclusion

Summary

Traduzione automatica

在这里, 我们提出了一个协议, 以表征核糖体粒子在单分子水平上使用静态和延时原子力显微镜 (afm) 成像技术。所描述的表面功能化方法允许在纳米尺度上以高分辨率捕获核糖体的结构和动力学。

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